JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞

產(chǎn)品型號(hào)：CRL-3006
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CRL-3006 JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞

ATCC^® Number:

CRL-3006™

Price:

$417.00

Designations:	JeKo-1
Depositors:	Elias Campo
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	suspension
Organism:	Homo sapiens (human)
Morphology:	lymphoblast Characterization Data
Source:	Organ: peripheral blood Tissue: Mantle Cell Lymphoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-3006 JeKo-1 人套細(xì)胞淋巴瘤細(xì)胞
Antigen Expression:	CD3-, CD5+, CD10+, CD19+ (verified at ATCC )
DNA Profile (STR):	Amelogenin:X CSF1PO:9,12 D13S317:8,9 D16S539:12 D5S818:10,13 D7S820:10,11 THO1:7 TPOX:8 vWA: 14
Age:	78
Gender:	female
Comments:	The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C
Subculturing:	Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.
Preservation:	Freeze medium: 30% RPMI; 60% FBS; 10% DMSO Storage temperature: liquid nitrogen vapor phase
References:	92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697 16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063

CRL-3006

ATCC^® Number:

CRL-3006™

Price:

$417.00

Designations:	JeKo-1
Depositors:	Elias Campo
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	suspension
Organism:	Homo sapiens (human)
Morphology:	lymphoblast Characterization Data
Source:	Organ: peripheral blood Tissue: Mantle Cell Lymphoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression:	CD3-, CD5+, CD10+, CD19+ (verified at ATCC )
DNA Profile (STR):	Amelogenin:X CSF1PO:9,12 D13S317:8,9 D16S539:12 D5S818:10,13 D7S820:10,11 THO1:7 TPOX:8 vWA: 14
Age:	78
Gender:	female
Comments:	The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C
Subculturing:	Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.
Preservation:	Freeze medium: 30% RPMI; 60% FBS; 10% DMSO Storage temperature: liquid nitrogen vapor phase
References:	92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365 16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697 16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063

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