BB7.2小鼠雜交瘤 B淋巴細胞

產品型號：HB-82
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細胞原代牛、狗、雞、鴨、魚原代細胞羊原代細胞豬原代細胞兔原代細胞小鼠原代細胞大鼠原代細胞人原代細胞培養基原代細胞凍存液 ATCC 人乳腺癌豬正常細胞豬睪丸細胞系人膠質瘤細胞人成骨細胞人永生化肝細胞人肝癌細胞人卵巢癌細胞人結直腸腺癌人經典小細胞肺癌細胞人小細胞肺癌細胞人結直腸腺癌細胞人鼻腔上皮細胞中國倉鼠卵巢懸浮細胞嗜性逆轉錄病毒包裝穩定細胞株小鼠神經細胞瘤人眼脈洛膜黑色素瘤 PT67 逆轉錄酶病毒包裝細胞鴨子胚胎成纖維細胞豬腎細胞系昆蟲細胞貓腎細胞恒河猴胚腎細胞倉鼠卵巢細胞負鼠腎細胞倉鼠肺細胞果蠅胚胎細胞家貓肺成纖維樣細胞雞胚細胞豚鼠心肌來源細胞豚鼠肺成纖維樣細胞豚鼠皮膚成纖維樣細胞狗腎細胞非洲綠猴胚胎腎上皮細胞猴胚胎腎上皮細胞長臂猿淋巴瘤新生牛眼囊成纖維細胞黑化大家鼠肺成纖維樣細胞俄勒岡地鼠細胞家兔皮膚成纖維樣細胞昆蟲卵巢細胞人胸膜瘤細胞非洲綠猴腎細胞 VERO 衍生株（SVP）白化金黃地鼠肺成纖維樣細胞黃胸鼠肺成纖維樣細胞白鰱尾鰭細胞蝙蝠肺細胞草魚腎細胞系大黃魚腎細胞猴腎細胞系犬腎細胞系人結腸直腸腺癌豬肺泡巨噬細胞 PFHSA05（TLL2）人重組特洛德樣蛋白因子 PFHSA04 （BMP1）人重組骨誘導因子人乳腺癌細胞結腸癌細胞成纖維細胞人胚腎細胞動物卵巢細胞動物胚胎細胞動物肺細胞肋骨來源細胞成纖維樣細胞動物上皮細胞動物肝臟細胞可誘導表達穩定細胞株動物氣管細胞動物腎細胞動物巨噬細胞人正常細胞人腫瘤細胞、癌細胞小鼠正常細胞小鼠腫瘤細胞、癌細胞大鼠正常細胞大鼠腫瘤細胞其它動物細胞查看全部產品

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HB-82 BB7.2小鼠雜交瘤 B淋巴細胞 的詳細介紹

HB-82 BB7.2小鼠雜交瘤 B淋巴細胞

ATCC^® Number:

HB-82™

Price:

$399.00

Designations:	BB7.2
Depositors:	P Parham
Isotype:	IgG2b
Biosafety Level:	1
Shipped:	frozen
Medium & Serum:	See Propagation
Growth Properties:	suspension
Organism:	Mus musculus （B cell）; Mus musculus （myeloma）（mouse （B cell）; mouse （myeloma））
Morphology:	lymphoblast
Source:	Cell Type: hybridoma: B lymphocyte;
Cellular Products:	immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxic
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HB-82
Comments:	Animals were immunized with papain solubilized HLA A2 antigen. Spleen cells were fused with NS-1 myeloma cells. Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding. [39935] BB7.2 and PA2.1 （ATCC HB-117） recognize overlapping epitopes on the alpha 2 domain of HLA-A2. [39936] The antibody cross-reacts with cells from various primate species. Tested and found negative for ectromelia virus （mousepox）.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References:	950: Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415 32263: Cole DJ, et al. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Cancer Res. 57: 5320-5327, 1997. PubMed: 9393756 39935: Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229 39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666

ATCC® Number: HB-82™ Price: $399.00

Designations: BB7.2

Depositors: P Parham

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus （B cell）; Mus musculus （myeloma）（mouse （B cell）; mouse （myeloma））

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxic

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with papain solubilized HLA A2 antigen.

Spleen cells were fused with NS-1 myeloma cells.

Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding. [39935]

BB7.2 and PA2.1 （ATCC HB-117） recognize overlapping epitopes on the alpha 2 domain of HLA-A2. [39936]

The antibody cross-reacts with cells from various primate species.

Tested and found negative for ectromelia virus （mousepox）.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 950: Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415

32263: Cole DJ, et al. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Cancer Res. 57: 5320-5327, 1997. PubMed: 9393756

39935: Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229

39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666

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BB7.2小鼠雜交瘤 B淋巴細胞